NanoMosaic Nanoneedle Assays for Robust Quantification and Determination of AAV Genome species packaged and Capsid Titers during Manufacturing Process
HIghlights of the Poster
- Short ITR adjacent DNA regions were significantly higher than those of the short central DNA region, indicating the prevalence of ITR adjacent truncations in all the preparations and suggesting that central region truncations without the ITRs are rare.
- Short fragment titers of the central DNA region (EGFP) are close to the full-length measurements and act as an acceptable surrogate proxy to the truly full-length transgene.
- The agreement between full-length measurements and the central regions, but not the edges, is specific to the viral genome sequence. Therefore, full-length measurements should be considered the gold standard for assessing AAV quality.
- The full-length viral-genome nanoneedle assays can provide direct measure of capsids containing complete genomes and can be used for improved assessment of AAV quality.
NanoMosaic Nanoneedle Assays for Robust Quantification and Determination of AAV Genome species packaged and Capsid Titers during Manufacturing Process
HIghlights of the Poster
- Short ITR adjacent DNA regions were significantly higher than those of the short central DNA region, indicating the prevalence of ITR adjacent truncations in all the preparations and suggesting that central region truncations without the ITRs are rare.
- Short fragment titers of the central DNA region (EGFP) are close to the full-length measurements and act as an acceptable surrogate proxy to the truly full-length transgene.
- The agreement between full-length measurements and the central regions, but not the edges, is specific to the viral genome sequence. Therefore, full-length measurements should be considered the gold standard for assessing AAV quality.
- The full-length viral-genome nanoneedle assays can provide direct measure of capsids containing complete genomes and can be used for improved assessment of AAV quality.