Case Study
Full-length & short-region transgene quantifications reveal truncation loci and improve product quality
Highlights
- Short-region and full-length transgene titers differ significantly for the CAG promoter, but not for CMV, suggesting substantial truncation in CAG-promoter samples.
- Multiplex nanoneedle technology quantifies heterogeneous AAV sub-populations, including truncated genomes, to pinpoint construct vulnerabilities.
- The truncation analysis is missed by conventional methods but confirmed by long-read sequencing.
- The full poster presents the comprehensive genome mapping of heterogeneous sub-populations within the AAV preparation.
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Case Study
Full-length & short-region transgene quantifications reveal truncation loci and improve product quality
Highlights
- Short-region and full-length transgene titers differ significantly for the CAG promoter, but not for CMV, suggesting substantial truncation in CAG-promoter samples.
- Multiplex nanoneedle technology quantifies heterogeneous AAV sub-populations, including truncated genomes, to pinpoint construct vulnerabilities.
- The truncation analysis is missed by conventional methods but confirmed by long-read sequencing.
- The full poster presents the comprehensive genome mapping of heterogeneous sub-populations within the AAV preparation.